E. COLI K antisera for counter current immunoelectrophoresis and slide agglutination
Most E. coli are enclosed in a capsule consisting of polysaccharides, also known as acid polysaccharides. The capsules can be variously shaped and be more or less heat stable.
E. coli K antisera consist of polyclonal antibodies produced in rabbits. The antisera are used for serotyping of the 67 known E. coli K antigens.
K antisera are polyclonal antibodies produced by immunising rabbits with whole formalin-killed cells expressing as much capsular polysaccharide as possible.
K antisera are supplied ready for use in 1 mL bottles, which is sufficient for at least 65 tests (50 tests for K9 antisera for slide agglutination).
We offer the full range of antisera for E. coli serotyping
All absorbed free of cross-reactions
We offer experienced technical support
How to use
K antisera are used for current counter immunoelectrophoresis and cannot be used for slide agglutination.
The only exception is K9 antisera, article number 84888, which can be used for slide agglutination for the EPEC strain O104:K9. K9 antisera for slide agglutination was developed during the O104:K9 outbreak in Germany in 2011, and is not for general serotyping of K9 capsules.
SSI Diagnostica has K antisera against all defined K antigens. The number of K antigens ranges from K2 to K103, but 32 numbers in the sequence are missing. This is because antigens, previously thought to be K antigens, have since then turned out not to be E. coli capsule antigens after all, but Klebsiella and E. coli fimbriae antigens, amongst others. The K antigens eventually proved not to be E. coli capsule antigens are K21, K58-K73, K75-K81, K86, K88, K90, K91, and K99.
E. coli K single antisera for slide agglutination
|1 mL vial
E. coli K single antisera for counter current immunoelectrophoresis, undiluted (for research use only)
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Antibodies can be frozen, but the process of freezing and thawing is damaging for the antibodies, and the titer will drop (even though not dramatically) due to decomposition.
SSI Diagnostica has not made further studies on this, and cannot guarantee the stability of the antisera, but we have experienced that the titer mostly stays at the same level if an antiserum is only frozen and thawn once.
We cannot guarantee that the titer of our antiserum is at the correct level when it has expired. Generally high-titer (undiluted) products keep the titer better than ready-to-use sera. Some antisera (especially H antisera) have a tendency to maintain the titer better than others (such as OK O86). Generally, some antisera can be used after the expiry date and some cannot. Since we cannot say which antisera that maintain the titer and which that do not, we recommend that you buy new ones. The problem in using expired antisera is that you will get false negatives without knowing it.
Yes, it is possible if you place an order in advance with the expected total volume for the period. In return we guarantee to keep the reserved lot for you.
Please send an inquiry. Depending on the needed volume, we might be able to develop the requested product. It can be anything from a new pool composition to a new antiserum.
Yes, you can buy all reference strains for the O, H and K antigens. We also have a database containing more than 62,000 wild type strains, which you can also buy.
If you buy ready-to-use sera, the sera can NOT be diluted.
The titer indicates how many times the serum can be diluted and still give a positive reaction.
The high titer products can be diluted according to the instructions listed on the packaging. The dilution rate varies from product to product, depending on how specific serum, the different rabbits have produced. If the high titer products are diluted more than stated on the packaging, SSI Diagnostica cannot guarantee that the antiserum captures all the wild type strains.
Antiserum consists primarily of IgG antibodies.
We recommend that you use the volume stated in the product insert. It is particularly important to note that if the dilution of the antiserum is too high it will not catch the weakest reactions. Further, you will experience a weak agglutination.
We offer a yearly training course. Contact us for more information.
A reference strain is a microorganism defined to at least the genus and species level, catalogued and described according to its characteristics e.g. O, K and H serotype and stating its origin.
A test strain is generally used for quality control and performance testing of culture media or serving as a positive/negative control in a diagnostic kit ensuring that the performance of the test has been successful.
Wild type strains are isolates from patients, animals or other sources, and these are not well defined.
It can ususally be done within three weeks.
You should send the strain according to your national legislation. The strain should be transported in an ordinary transport medium (Stuarts, Cary Blair, lyophilised or likewise), and the package should be marked with an UN number corresponding to the content of the package.
The main advantages are:
1. We offer the full range of antisera for E. coli serotyping.
2. Reaction time for OK O antisera is only 10 seconds reducing the risk of false positive reactions leading to a more accurate result compared to other tests having a reaction time of 1 minute.
3. SSI antisera are absorbed and thus very specific.
4. We offer technical expertise and assistance / training.
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