DEC PCR Kit

The DEC PCR Kit is a multiplex PCR system that detects virulence genes from diarrhoeagenic E. coli 1 (DEC). Traditionally, this has been achieved by serotyping, followed by verocytotoxin cell assays, and therefore strains have previously been overlooked where they have virulence genes that have not had a serotype known for its pathogenicity. The DEC Primer Kit therefore makes it possible to identify more precisely pathogenic E. coli strains. The kit contains a primer mix that consists of 8 primer pairs aimed at the following genes: intimin (eae), verocytotoxin 1 and 2 (vtx1 and vtx2, also called stx1 and stx2), heat-stable enterotoxin, human variant (estAhuman) and porcine variant (estAporcine), heat-stable enterotoxin (eltA), invasive plasmid antigen (ipaH) and 16S rDNA (functions as an internal positive control).

The most important groups of diarrhoeagenic E. coli strains and the genes used to determine them are set out below: 

  • Verocytotoxin-producing strains (VTEC) – identification: demonstration of vtx1 and vtx2.  
  • “Attaching and effacing” Ecoli (A/EEC) including enteropathogen Ecoli(EPEC) – identification: demonstration of eae.  
  • Enterotoxigenic Ecoli (ETEC) – identification: demonstration of eltAestAhuman, or estAporcine.  
  • Enteroinvasive Ecoli (EIEC) – identification: demonstration of ipaH.  

The DEC PCR Kit therefore allows identification of all of these groups.

Product description

The kit contains all necessary reagents to carry out the DEC PCR reactions.

The kit contains: TE buffer and 10% Chelex-100 in TE buffer to prepare the template, primer mix, PCR Ready Mix (including loading buffer) and two PCR-positive DNA controls.

There is enough reagent for 100 PCR reactions in the kit.

The DEC PCR Kit is a multiplex PCR system that detects virulence genes from diarrhoeagenic E. coli 1 (DEC)

The product can be used in all microbiological laboratories that detect E. coli

The kit contains reagent enough for 100 tests

The kit contains all necessary reagents to carry out the DEC PCR reactions.

How to use

The DEC PCR Kit is used for in vitro diagnostics of diarrhoeagenic E. coli.

The product can be used in all microbiological laboratories that detect E. coli.

Product range

Our DEC PCR is sold also as DEC Primer Mix, which includes only the primer mix plus two PCR-positive DNA controls.

Article
number 
Product Number
of tests
Packing 
73410 DEC 
PCR-kit 
100 1 box 

References

1 S. Persson, K.E.P. Olsen, F. Scheutz, K.A. Krogfelt and P. Gerner-Smidt. A method for fast and simple detection of major diarrhoeagenic Escherichia coliin the routine diagnostic laboratory. Clin Microbiol Infect 2007; 13: 516-524

Downloads

Please find the available downloads here.

IFU

Click on the link below to download the IFU.

PCR

Contact

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    E. coli Antisera

    SSI Diagnostica offer E.coli antisera for full serotyping of all O, K and H antigens plus the most frequent bacteriophages and F antigens. 

    An important tool in diagnosis, monitoring and testing

    Over the last few decades, a number of new E. coli pathogens have been identified, e.g. VTEC (verocytotoxin-producing E. coli) and EPEC (enteropathogenic E. coli). This has increased the need for E. coli serotyping, as an important tool in diagnosis, monitoring and testing.

    Antisera from SSI Diagnostica are for in vitro use in human, veterinary and food laboratories. All antisera are produced in accordance with internationally recognised methods dating back to the time of Fritz Kauffmann, Ida Ørskov and Frits Ørskov, combined with the knowledge accumulated since then.

    All antisera are polyclonal and are produced by immunising rabbits with well-defined reference strains. For antisera, wherever practicable, the reaction is made specific by absorption. All finished products are tested against a large number of strains.

    • Comprehensive antisera range to serotype all know E. coli strains
    • High quality and technical support
    • CE-marked (except for the E. coli K antisera)

    E.coli Antisera Solutions

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    E. coli H antisera consist of polyclonal antibodies produced in rabbits. The antisera are used for serotyping af the 53 known E. coli H antigens. The agglutination assay provides a clear and precise result within two hours.

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    E. coli K antisera consist of polyclonal antibodies produced in rabbits. The antisera are used for serotyping of the 67 known E. coli K antigens.

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    Contact

    Please, reach out if you have questions, comments or requests.