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Biological Indicators

Biological indicators for validation of your sterilisation process – for steam, dryheat, and formaldehyde

Biological Indicators, how to use

Biological indicators used to test steam sterilisation are microorganisms (spores of Geobacillus stearothermophilus, originally from ATCC 7953), prepared on filter paper as carrier material and packed in primary packaging consisting of transparent plastic film and paper which is steam-permeable.

The steam indicators fulfil the specifications in EN ISO 11138-3, i.e. an initial microbial count of at least 105 CFU/unit, a D-value at 121°C of at least 1.5 min. and a Z-value of at least 6°C.

  • Certify your sterilisation process
  • Easy workflow
  • Validation possible for autoclaves, industrial dishwashers and more

Make sure sterile is actually sterile

By using biological indicators you validate that all microorganisms have been killed during the process.

Biological indicators are living bacterial spores, which are resistant to high temperatures and non-pathogenic. If those bacteria is killed in your process no other bacteria, spores or fungi will be able to live either.

Biological Indicator Solutions

Biological Indicator Steam

Steam sterilisation (or autoclaving) is carried out by introducing saturated water vapour under pressure. In addition to the temperature effect itself, the presence of saturated water vapour is essential for killing microorganisms, their destruction being due to irreversible denaturation of cell components.

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Biological Indicator Dry Heat

Sterilisation with dry heat (dry sterilisation) takes place in an oven, in which the air must be able to circulate. It is the direct temperature effect, i.e. the irreversible denaturation of cell components, which destroys microorganisms present.

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Biological Indicators – Dual Incubator

Incubator for Biological Indicators and hygiene monitoring systems can be used for Biological Indicators ampoules and for culture mediums.

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Order Indicators Here

 With a biological and chemical indicators subscription, you:

  • Will receive certificates for approved test results
  • Can have the biological indicators cultured by our certified laboratory
  • Ensure safety for your patients and staff
  • Have a subscription scheme so you always have the indicators you need
  • Receive personal guidance and free biological indicators for re-testing test-positives
OBS: Subscriptions done on this page is only for Danish customers. If you are from outside Denmark please contact your sales rep. or write an email to

Other interesting content

Looking for something else? You might be interested in this as well.

Chemical Indicator, for your daily control

Your daily quality check of your sterilisation process.

Checking your temperature and time was sufficient and easy reading of the test.

Read more

Cleaning indicator for verification of your industrial dishwasher

SSI Diagnostica provides several types of indicators. This includes ampules for self testing, indicators for visual cleaning of hospital beds and indicators for laundry. Further more we hold a portfolio of process challenges devices for cleaning indication.

Read more

Get in touch

Please, reach out if you have a question, comment or request.