Pneumococcus Antisera for Neufeld
Pneumococci are enclosed within a capsule consisting of repeated units of polysaccharides. The composition of the polysaccharides defines the serotype, and 91 pneumococcal serotypes have been described to date, with more on the way. The 91 serotypes are divided into 25 types and 21 groups. Each group consists of 2-5 serotypes which are very similar.
Product description
Pool, type and factor antisera are produced by immunising rabbits with killed whole cells. Pool antisera are a mixture of antisera. Pools A-I contain antisera against all serotypes, while pools P‑T are mixtures of antisera against the 23-valent vaccine serotypes. By combining the two Pool systems (the Pneumotest products), the 23 serotypes can be serotyped easily to type/group level.
Contains antisera against
Pool A, type 1, 2, 4, 5, group 18
Pool B, type 3, 8, group 6, 19
Pool C, type 20, 31, 40, group 7, 24
Pool D, type 36, 37, group 9, 11, 16
Pool E, type 21, 39, group 10, 12, 33
Pool F, type 27, group 17, 22, 32, 41
Pool G, type 29, 34, 42, group 35, 47
Pool H, type 13, 14, group 15, 23, 28
Pool I, type 38, 43, 44, 45, 46, 48, group 25
Pool P, type 1, 14, group 7, 19
Pool Q, group 6, 18, 23
Pool R, type 3, 4, group 9, 12
Pool S, type 5, 8, group 10, 15, 17
Pool T, type 2, 20, group 11, 22, 33
Type antisera react specifically with the serotype in question.
Group antisera are specific antisera reacting with all serotypes in the group in question. The ultimate identification of the serotypes in the group is carried out by serotyping with factor antisera. The combination of the results defines the serotype.
All the products are absorbed free of cross-reactions, but factor sera are absorbed free of cross‑reactions only within the group. In the individual cases below, it is not possible to produce a specific antiserum without losing titre of the homologous antibody. Therefore, the following cross-reactions will occur:
Antiserum cross-reacting type
Group 25: 38
Type 29: 35B
Group 35: 42, 47F
Type 42: 20, 31 33A, 35A, 35B, 35C
Each product is supplied in a vial with 1 ml ready-to-use antiserum, sufficient for approx. 300 tests.
Many years of experience producing Pneumococcus antisera
High quality
All absorbed free of cross-reactions
We offer experienced technical support
How to use
Pool, type, group and factor antisera are used in capsular reactions, also called the Neufeld test. Equal quantities of antiserum and bacterial culture are mixed on a slide. Under a phase contrast microscope, a positive reaction is indicated when the capsule enclosing the pneumococcus swells and becomes visible. The bacteria will frequently also agglutinate.
Product range
The 14 pools can be bought individually, or 12 of them can be bought together in the Pneumotest-kit. There are 25 type antisera, 21 group antisera and 64 factor antisera.
Pool antisera
| Article number | Product | React with type: | Number of tests | Packing |
|---|---|---|---|---|
| 16725 | Pool A | 1,2,4, 5,18F,18A, 18B,18C | 300 | 1 mL vial |
| 16728 | Pool B | 3,6A,6B, 6C,6D,8,19F,19B, 19C | 300 | 1 mL vial |
| 16730 | Pool C | 7F,7A,7B, 7C,20,24F, 24A,24B,31, 40 | 300 | 1 mL vial |
| 16731 | Pool D | 9A,9L,9N, 9V,11F,11A, 11B,11C,11D, 16F,16A,36, 37 | 300 | 1 mL vial |
| 16733 | Pool E | 10F,10A,10B, 10C,12F,12A, 12B,21,33F, 33A,33B,33C, 33D,39 | 300 | 1 mL vial |
| 16734 | Pool F | 17F,17A,22F, 22A,27,32F, 32A,41F,41A | 300 | 1 mL vial |
| 16735 | Pool G | 29,34,35F, 35A,35B,35C, 42,47F,47A | 300 | 1 mL vial |
| 16736 | Pool H | 13,14,15F, 15A,15B,15C, 23F,23A,23B, 28F,28A | 300 | 1 mL vial |
| 16737 | Pool I | 25F,25A,38, 43,44,45, 46,48 | 300 | 1 mL vial |
| 16739 | Pool P | 1,7F,7A, 7B,7C,14, 19F,19A,19B, 19C | 300 | 1 mL vial |
| 16740 | Pool Q | 6A,6B,6C, 6D,18F,18A,18B, 18C,23F,23A, 23B | 300 | 1 mL vial |
| 16741 | Pool R | 3,4,9A, 9L,9N,9V 12F,12A,12B | 300 | 1 mL vial |
| 16742 | Pool S | 5,8,10F, 10A,10B,10C, 15F,15A,15B, 15C,17F,17A | 300 | 1 mL vial |
| 16743 | Pool T | 2,11F,11A, 11B,11C,11D, 20,22F,22A, 33F,33A,33B, 33C,33D | 300 | 1 mL vial |
Type antisera
| Article number | Product | Incl. in pool | Number of tests | Packing |
|---|---|---|---|---|
| 16744 | Type 1 | A,P | 300 | 1 mL vial |
| 16745 | Type 2 | A,T | 300 | 1 mL vial |
| 16746 | Type 3 | B,R | 300 | 1 mL vial |
| 16747 | Type 4 | A,R | 300 | 1 mL vial |
| 16748 | Type 5 | A,S | 300 | 1 mL vial |
| 16751 | Type 8 | B,S | 300 | 1 mL vial |
| 16752 | Type 13 | H | 300 | 1 mL vial |
| 16753 | Type 14 | H,P | 300 | 1 mL vial |
| 16754 | Type 20 | C,T | 300 | 1 mL vial |
| 16755 | Type 21 | E | 300 | 1 mL vial |
| 16756 | Type 27 | F | 300 | 1 mL vial |
| 16757 | Type 29 | G | 300 | 1 mL vial |
| 16758 | Type 31 | C | 300 | 1 mL vial |
| 16759 | Type 34 | G | 300 | 1 mL vial |
| 16760 | Type 36 | D | 300 | 1 mL vial |
| 16761 | Type 37 | D | 300 | 1 mL vial |
| 16762 | Type 38 | I | 300 | 1 mL vial |
| 16763 | Type 39 | E | 300 | 1 mL vial |
| 16764 | Type 40 | C | 300 | 1 mL vial |
| 16765 | Type 42 | G | 300 | 1 mL vial |
| 16766 | Type 43 | I | 300 | 1 mL vial |
| 16767 | Type 44 | I | 300 | 1 mL vial |
| 16769 | Type 45 | I | 300 | 1 mL vial |
| 16770 | Type 46 | I | 300 | 1 mL vial |
| 16771 | Type 48 | I | 300 | 1 mL vial |
Group antisera
| Article number | Product | React with type: | Incl. in pool | Number of tests | Packing |
|---|---|---|---|---|---|
| 16900 | Group 6 | 6A,6B,6C,6D | B,Q | 300 | 1 mL vial |
| 16902 | Group 7 | 7F,7A,7B, 7C | C,P | 300 | 1 mL vial |
| 16903 | Group 9 | 9A,9L,9N, 9V | D,R | 300 | 1 mL vial |
| 16904 | Group 10 | 10F,10A,10B, 10C | E,S | 300 | 1 mL vial |
| 16905 | Group 11 | 11F,11A,11B, 11C,11D | D,T | 300 | 1 mL vial |
| 16906 | Group 12 | 12F,12A,12B | E,R | 300 | 1 mL vial |
| 16907 | Group 15 | 15F,15A,15B, 15C | H,S | 300 | 1 mL vial |
| 16908 | Group 16 | 16F,16A | D | 300 | 1 mL vial |
| 16909 | Group 17 | 17F,17A | F,S | 300 | 1 mL vial |
| 16910 | Group 18 | 18F,18A,18B, 18C | A,Q | 300 | 1 mL vial |
| 16911 | Group 19 | 19F,19A,19B, 19C | B,P | 300 | 1 mL vial |
| 16912 | Group 22 | 22F,22A | F,T | 300 | 1 mL vial |
| 16913 | Group 23 | 23F,23A,23B | H,Q | 300 | 1 mL vial |
| 16914 | Group 24 | 24F,24A,24B | C | 300 | 1 mL vial |
| 16915 | Group 25 | 25F,25A | I | 300 | 1 mL vial |
| 16916 | Group 28 | 28F,28A | H | 300 | 1 mL vial |
| 16917 | Group 32 | 32F,32A | F | 300 | 1 mL vial |
| 16918 | Group 33 | 33F,33A,33B, 33C,33D | E,T | 300 | 1 mL vial |
| 16919 | Group 35 | 35F,35A,35B, 35C | G | 300 | 1 mL vial |
| 16920 | Group 41 | 41F,41A | F | 300 | 1 mL vial |
| 16921 | Group 47 | 47F,47A | G | 300 | 1 mL vial |
Factor antisera
| Article number | Product | React with type: | Number of tests | Packing |
|---|---|---|---|---|
| 16922 | Factor 6a | 33D | 300 | 1 mL vial |
| 16923 | Factor 6b | 6A | 300 | 1 mL vial |
| 16924 | Factor 6c | 6B,6D | 300 | 1 mL vial |
| 73266 | Factor 6d | 6C,6D | 300 | 1 mL vial |
| 16925 | Factor 7b | 7F,(7A) | 300 | 1 mL vial |
| 16926 | Factor 7c | 7A | 300 | 1 mL vial |
| 16927 | Factor 7e | 7B | 300 | 1 mL vial |
| 16931 | Factor 7f | 7C | 300 | 1 mL vial |
| 16933 | Factor 7h | 19B,19C | 300 | 1 mL vial |
| 16934 | Factor 9b | 9L,9N | 300 | 1 mL vial |
| 16935 | Factor 9d | 9A,9V | 300 | 1 mL vial |
| 16936 | Factor 9e | 9N | 300 | 1 mL vial |
| 16937 | Factor 9g | 9V | 300 | 1 mL vial |
| 16939 | Factor 10b | 10F,10B,10C | 300 | 1 mL vial |
| 16940 | Factor 10d | 10A,10B | 300 | 1 mL vial |
| 16941 | Factor 10f | 10C | 300 | 1 mL vial |
| 16942 | Factor 11b | 11F,11B,11C, 11D | 300 | 1 mL vial |
| 16944 | Factor 11c | 11A,11C,11D | 300 | 1 mL vial |
| 16945 | Factor 11f | 11B,11C | 300 | 1 mL vial |
| 16946 | Factor 11g | 11F,11B | 300 | 1 mL vial |
| 16947 | Factor 12b | 12F,12B | 300 | 1 mL vial |
| 16948 | Factor 12c | 12A,12B | 300 | 1 mL vial |
| 16949 | Factor 12e | 12B | 300 | 1 mL vial |
| 16950 | Factor 15b | 15F,15B | 300 | 1 mL vial |
| 16951 | Factor 15c | 15F,15A | 300 | 1 mL vial |
| 16952 | Factor 15e | 15B,15C | 300 | 1 mL vial |
| 16953 | Factor 15h | 15B | 300 | 1 mL vial |
| 16954 | Factor 16b | 16F | 300 | 1 mL vial |
| 16955 | Factor 16c | 16A | 300 | 1 mL vial |
| 16956 | Factor 17b | 17F | 300 | 1 mL vial |
| 16957 | Factor 17c | 17A | 300 | 1 mL vial |
| 16958 | Factor 18c | 18F,18C | 300 | 1 mL vial |
| 16959 | Factor 18d | 18A | 300 | 1 mL vial |
| 16960 | Factor 18e | 18F,18B,18C | 300 | 1 mL vial |
| 16961 | Factor 18f | 18F | 300 | 1 mL vial |
| 16962 | Factor 19b | 19F | 300 | 1 mL vial |
| 16963 | Factor 19c | 19A | 300 | 1 mL vial |
| 16964 | Factor 19f | 19C | 300 | 1 mL vial |
| 16965 | Factor 20b | 33A | 300 | 1 mL vial |
| 16966 | Factor 22b | 22F | 300 | 1 mL vial |
| 16967 | Factor 22c | 22A | 300 | 1 mL vial |
| 16968 | Factor 23b | 23F | 300 | 1 mL vial |
| 16969 | Factor 23c | 23A | 300 | 1 mL vial |
| 16970 | Factor 23d | 23B | 300 | 1 mL vial |
| 16971 | Factor 24c | 24A | 300 | 1 mL vial |
| 16972 | Factor 24d | 24F,24A | 300 | 1 mL vial |
| 16973 | Factor 24e | 24B | 300 | 1 mL vial |
| 16974 | Factor 25b | 25F | 300 | 1 mL vial |
| 16975 | Factor 25c | 25A | 300 | 1 mL vial |
| 16976 | Factor 28b | 28F | 300 | 1 mL vial |
| 16977 | Factor 28c | 28A | 300 | 1 mL vial |
| 16978 | Factor 29b | 35B | 300 | 1 mL vial |
| 16979 | Factor 32a | 32F,32A | 300 | 1 mL vial |
| 16980 | Factor 32b | 32A | 300 | 1 mL vial |
| 16981 | Factor 33b | 33F,33A | 300 | 1 mL vial |
| 16982 | Factor 33e | 33C | 300 | 1 mL vial |
| 16983 | Factor 33f | 33B,(33C),33D | 300 | 1 mL vial |
| 16984 | Factor 35a | 35F,35A,35B,35C | 300 | 1 mL vial |
| 16986 | Factor 35b | 35F | 300 | 1 mL vial |
| 16992 | Factor 35c | 35A,35B,35C | 300 | 1 mL vial |
| 16994 | Factor 41a | 41F,41A | 300 | 1 mL vial |
| 16995 | Factor 41b | 41F | 300 | 1 mL vial |
| 16996 | Factor 42a | 35C | 300 | 1 mL vial |
| 16997 | Factor 43b | 47A | 300 | 1 mL vial |
| 16998 | Factor 47a | 47F,47A | 300 | 1 mL vial |
F.A.Q.
Click the question to get your answer
Antibodies can be frozen, but the process of freezing and thawing is damaging for the antibodies, and the titer will drop (even though not dramatically) due to decomposition.
We have not made further studies on this, and cannot guarantee the stability of the sera, but we have experienced that mostly the titer stays at the same level if a serum only is frozen and thawn once.
Yes, it is possible, if you place an order in advance with the expected total volume for the period. In return we guarantee to keep the reserved lot for you.
Depending on the needed volume, we might be able to develop the requested product. It can be anything from a new pool composition to a new serum.
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