Dermatophyte PCR Kit
Nail infections are caused chiefly by T. rubrum and T. mentagrophytes. Traditionally, these species are identified from nail samples by culturing on medium, which takes 10-15 days to 3-4 weeks. The Dermatophyte PCR Kit is a multiplex PCR system that enables determination of dermatophytes generally and T. rubrum from nail samples within 5 hours. The primer mix contains two primer pairs aimed at, respectively, chitin synthase 1 for detection of dermatophytes generally and ITS2 (internal transcribed spacer) to detect T. rubrum. Furthermore, the primer mix contains an internal plasmid control that functions as a template for the primer pair specifically for T. rubrum.
By comparing the dermatophyte multiplex PCR method with conventional methods of identifying nail infections, it has been shown that the PCR method identifies most dermatophyte and T. rubrum-positive nail samples (ref. 1, 2 and 3).
The kit contains all reagents necessary to carry out dermatophyte PCR reactions.
The kit contains Buffer A and Buffer B to prepare the template, primer mix and PCR Ready Mix (including loading buffer) to run the samples and two PCR-positive DNA controls—one containing dermatophyte genomic DNA and one containing T. rubrum genomic DNA.
There is enough reagent for 100 PCR reactions in the kit.
The Dermatophyte PCR Kit is a multiplex PCR system that enables determination of dermatophytes generally and T. rubrum from nail samples within 5 hours
The kit contains all reagents necessary to carry out dermatophyte PCR reactions
There is enough reagent for 100 PCR reactions in the kit
The Dermatophyte PCR Kit is used for in vitro diagnostics of dermatophytes in general and T. rubrum from nail samples
How to use
The Dermatophyte PCR Kit is used for in vitro diagnostics of dermatophytes in general and T. rubrum from nail samples.
1. A. Brillowska-Dabrowska, D. M. Saunte and Maiken Cavling Arendrup. Five-hour diagnosis of dermatophyte nail infection with specific detection of Trichophyton rubrum. Jour Clin Microbiol (2007) 1200-1204.
2. N. Kondori, A.-L. Abrahomsson, N. Ataollahy and C. Wennerås. Comparison of a new commercial test, Dermatophyte-PCR kit, with conventional methods for rapid detection and identification of Trichophyton rubrum in nail specimens. Medical Mycology (2010), Early Online, 1-4.
3. A. Brillowska-Dabrowska, S. S. Nielsen, H. V. Nielsen and M. C. Arendrup. Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory. Medical Mycology 2010, 48, 828-831
4. Nisha Suyien Chandran, Jiun-Yit Pan,Zacharias AD Pramono, Hiok-Hee Tan and
Chew-Swee. Complementary role of a polymerase chain reaction test in the diagnosis of onychomycosis. Australasian Journal of Dermatology (2013) 54, 105–108
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