E. COLI BACTERIOPHAGES
In addition to our diagnostic antisera, SSI Diagnostica also sells specially produced bacteriophages for phage typing.
Bacteriophages are viruses that infect bacteria. Phage typing is used to type K1, K5, and K13 capsules, as specific antibodies cannot be produced against these. Phages are epitope-specific in relation to specific antigens, and these can therefore be used for serotyping. Phage typing is easy to perform and can under normal circumstances be carried out within 24 hours.
Bacteriophages are supplied in the form of 1 mL ready-to-use solution, sufficient for at least 600 tests.
E. coli cells infected with K1, K5, or K13 bacteriophages are propagated and concentrated, and then the bacteriophages are harvested. K1, K5, and K13 bacteriophages are sold as ready-to-use suspensions.
We offer the full range of antisera for E. coli serotyping
All absorbed free of cross-reactions
We offer experienced technical support
Shelf-life of 4 years
How to use
A streak of phage solution (K1, K5, and K13) is deposited diagonally on an agar plate. A streak of the bacterial culture to be typed is deposited vertically. After incubation overnight at 37°C, the plate is read. If the typing culture is infected with phages, the culture is K1, K5, and K13 positive.
SSI Diagnostica supplies K1, K5, and K13 bacteriophages for K-typing.
|60758||K1 bacteriophage||600||1 mL vial|
|60759||K5 bacteriophage||600||1 mL vial|
|55348||K13 bacteriophage||600||1 mL vial|
Click the question to get your answer
Yes, it is possible if you place an order in advance with the expected total volume for the period. In return we guarantee to keep the reserved lot for you.
Please send an inquiry. Depending on the needed volume, we might be able to develop the requested product. It can be anything from a new pool composition to a new antiserum.
Yes, you can buy all reference strains for the O, H and K antigens. We also have a database containing more than 62,000 wild type strains, which you can also buy.
We recommend that you use the volume stated in the product insert. It is particularly important to note that if the dilution of the antiserum is too high it will not catch the weakest reactions. Further, you will experience a weak agglutination.
We offer a yearly training course. Contact us for more information.
A reference strain is a microorganism defined to at least the genus and species level, catalogued and described according to its characteristics e.g. O, K and H serotype and stating its origin.
A test strain is generally used for quality control and performance testing of culture media or serving as a positive/negative control in a diagnostic kit ensuring that the performance of the test has been successful.
Wild type strains are isolates from patients, animals or other sources, and these are not well defined.
SSI can usually do this within three weeks.
You should send the strain according to your national legislation. The strain should be transported in an ordinary transport medium (Stuarts, Cary Blair, lyophilised or likewise), and the package should be marked with an UN number corresponding to the content of the package.
The main advantages are:
1. We offer the full range of antisera for E. coli serotyping.
2. Reaction time for OK O antisera is only 10 seconds reducing the risk of false positive reactions leading to a more accurate result compared to other tests having a reaction time of 1 minute.
3. SSI antisera are absorbed and thus very specific.
4. We offer technical expertise and assistance / training.
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