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66446S. sonnei phase I antiserum
66447S. sonnei phase I antiserum
66448S. sonnei phase I antiserum

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    Shigella Sonnei Fase Antisera

    S. sonnei consists of two phases. The two phases can be differentiated morphologically, as phase I occurs in the form of smooth colonies, whilst phase II occurs in the form of rough colonies. It is therefore crucial that a suitable culturing medium is chosen, as the transformation between the two phases is dependent on, among other factors, the concentration of divalent cations. The higher the concentration of divalent cations, the more rough colonies there are (phase II).

    A patient sample usually contains both types of colony.

    The antigen that identifies a phase II colony is a subset of the antigen that identifies the phase I colonies. Phase I colonies will therefore agglutinate in phase I sera, but some phase I strains will also agglutinate in phase II sera. Phase II colonies will agglutinate only in phase II sera.

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    How to use

    The Shigella strain is cultured overnight on a selective or a non-selective agar plate. A small colony is transferred to a slide and mixed with a drop of antiserum, and the slide is tilted slowly back and forth. If the bacterial colony has antigens that are homologous with the antiserum used, the cells will form antigen-antibody bonds, and clear aggregates will appear (agglutination). The result is read within 5-10 seconds of mixing. The reading is easiest to perform and clearest if the slide is held up in front of a light source against a black background.

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    How to use

    The Shigella strain is cultured overnight on a selective or a non-selective agar plate. A small colony is transferred to a slide and mixed with a drop of antiserum, and the slide is tilted slowly back and forth. If the bacterial colony has antigens that are homologous with the antiserum used, the cells will form antigen-antibody bonds, and clear aggregates will appear (agglutination). The result is read within 5-10 seconds of mixing. The reading is easiest to perform and clearest if the slide is held up in front of a light source against a black background.

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